听力与言语-语言病理学

行为科学

医学伦理学

你正在浏览JOURNAL OF EXPERIMENTAL BOTANY期刊下所有文献
  • Variation in the shoot calcium content of angiosperms.

    abstract::This study describes the variation in the mean relative shoot Ca content within the angiosperms at the ordinal level. Data were derived from studies in the literature in which the shoot Ca content of two or more species had been compared, and from a hydroponic experiment in which plants were selected to represent the ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg143

    authors: Broadley MR,Bowen HC,Cotterill HL,Hammond JP,Meacham MC,Mead A,White PJ

    更新日期:2003-05-01 00:00:00

  • Expression of a putative grapevine hexose transporter in tobacco alters morphogenesis and assimilate partitioning.

    abstract::Tobacco plants were transformed by leaf disc regeneration with the VvHT1 (Vitis vinifera hexose transporter 1) cDNA under the control of the constitutive CaMV 35S promoter in a sense or antisense orientation. Among the 20 sense plants and 10 antisense plants obtained, two sense plants showed a mutant phenotype when gr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg119

    authors: Leterrier M,Atanassova R,Laquitaine L,Gaillard C,Coutos-Thévenot P,Delrot S

    更新日期:2003-04-01 00:00:00

  • Fatty acid elongation is important in the activity of thiocarbamate herbicides and in safening by dichlormid.

    abstract::The thiocarbamates, such as pebulate (S-propyl butyl (ethyl) thiocarbamate) are a well-established class of herbicides. They inhibit fatty acid elongation, which is necessary for the biosynthesis of constituents of surface waxes and suberin and this has been proposed to be important for their toxicity. In this study l...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg126

    authors: Baldwin A,Rogers HJ,Francis D,Harwood JL

    更新日期:2003-04-01 00:00:00

  • A comparison of manual and automated systems for soil CO2 flux measurements: trade-offs between spatial and temporal resolution.

    abstract::Soil respiration is affected by distributions of roots and soil carbon substrates and by temperature and soil water content, all of which vary spatially and temporally. The objective of this paper was to compare a manual system for measuring soil respiration in a temperate forest, which had a greater spatial distribut...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg121

    authors: Savage KE,Davidson EA

    更新日期:2003-03-01 00:00:00

  • One-step analysis of seed storage data and the longevity of Arabidopsis thaliana seeds.

    abstract::Seeds of two ecotypes of Arabidopsis thaliana, NW20 and N1601, were aged over a range of saturated salt solutions at temperatures between 6 degrees C and 55 degrees C. For each ecotype, the results from 37 storage experiments were summarized using the Ellis and Roberts viability equations and a modified version of the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg103

    authors: Hay FR,Mead A,Manger K,Wilson FJ

    更新日期:2003-03-01 00:00:00

  • Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum.

    abstract::Genes encoding pathogenesis-related (PR-) proteins isolated from a cDNA library of Fusarium graminearum-infected wheat spikes of scab-resistant cultivar 'Sumai-3' were transformed into susceptible spring wheat, 'Bobwhite' using a biolistic transformation protocol, with the goal of enhancing levels of resistance agains...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg110

    authors: Anand A,Zhou T,Trick HN,Gill BS,Bockus WW,Muthukrishnan S

    更新日期:2003-03-01 00:00:00

  • Arabidopsis mutants sensitive to gamma radiation include the homologue of the human repair gene ERCC1.

    abstract::Mutants sensitive to ionizing radiation in yeast and mammals include an assortment of DNA repair genes. The majority of these DNA repair genes are involved in the repair of DNA double-strand breaks. In this study a forward genetic screen is used to identify gamma-sensitive mutants of Arabidopsis thaliana. The gamma-pl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg069

    authors: Hefner E,Preuss SB,Britt AB

    更新日期:2003-02-01 00:00:00

  • Ethylene is required for both the initiation and progression of softening in pear (Pyrus communis L.) fruit.

    abstract::In order to investigate the physiological role of ethylene in the initiation and subsequent progression of softening, pear fruit were treated with propylene, an analogue of ethylene or 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene action at the preclimacteric or ripening stages. The propylene treatment...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg073

    authors: Hiwasa K,Kinugasa Y,Amano S,Hashimoto A,Nakano R,Inaba A,Kubo Y

    更新日期:2003-02-01 00:00:00

  • Photosynthetic performance of an Arabidopsis mutant with elevated stomatal density (sdd1-1) under different light regimes.

    abstract::In the Arabidopsis mutant sdd1-1, a point mutation in a single gene (SDD1) causes specific alterations in stomatal density and distribution. In comparison to the wild type (C24), abaxial surfaces of sdd1-1 rosette leaves have about 2.5-fold higher stomatal densities. This mutant was used to study the consequence of st...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg087

    authors: Schlüter U,Muschak M,Berger D,Altmann T

    更新日期:2003-02-01 00:00:00

  • Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana.

    abstract::This article reviews the historical development of cytology and cytogenetics in Arabidopsis, and summarizes recent developments in molecular cytogenetics, with special emphasis on meiotic studies. Despite the small genome and small chromosomes of Arabidopsis, considerable progress has been made in developing appropria...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erg034

    authors: Armstrong SJ,Jones GH

    更新日期:2003-01-01 00:00:00

  • The actin cytoskeleton is a target of the self-incompatibility response in Papaver rhoeas.

    abstract::The integration of signals received by a cell, and their transduction to targets, is essential for all cellular responses. The cytoskeleton has been identified as a major target of signalling cascades in both animal and plant cells. Self-incompatibility (SI) in Papaver rhoeas involves an allele-specific recognition be...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erg003

    authors: Staiger CJ,Franklin-Tong VE

    更新日期:2003-01-01 00:00:00

  • Genetic approaches to understanding sugar-response pathways.

    abstract::Plants as photoautotrophic organisms are able to produce the carbohydrates they require and have developed mechanisms to co-ordinate carbohydrate production and its metabolism. Carbohydrate-derived signals regulate the expression of genes involved in both photosynthesis and metabolism, and control carbohydrate partiti...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erg054

    authors: Rook F,Bevan MW

    更新日期:2003-01-01 00:00:00

  • Function and specificity of 14-3-3 proteins in the regulation of carbohydrate and nitrogen metabolism.

    abstract::Protein phosphorylation is key to the regulation of many proteins. Altered protein activity often requires the interaction of the phosphorylated protein with a class of "adapters" known as 14-3-3 proteins. This review will cover aspects of 14-3-3 interaction with key proteins of carbon and nitrogen metabolism such as ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erg057

    authors: Comparot S,Lingiah G,Martin T

    更新日期:2003-01-01 00:00:00

  • Expression of a cyanobacterial sucrose-phosphate synthase from Synechocystis sp. PCC 6803 in transgenic plants.

    abstract::Sucrose-phosphate synthase (SPS) from the cyanobacterium Synechocystis sp. PCC 6803 lacks all of the Ser residues known to be involved in the regulation of higher plant SPS by protein phosphorylation. The Synechocystis SPS is also not allosterically regulated by glucose 6-phosphate or orthophosphate. To investigate th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg023

    authors: Lunn JE,Gillespie VJ,Furbank RT

    更新日期:2003-01-01 00:00:00

  • Biosensor reporting of root exudation from Hordeum vulgare in relation to shoot nitrate concentration.

    abstract::The aim of this study was to determine the relationship between shoot nitrate concentration, mediated by nitrate supply to roots, and root exudation from Hordeum vulgare. Plants were grown for 14 d in C-free sand microcosms, supplied with nutrient solution containing 2 mM nitrate. After this period, three treatments w...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg017

    authors: Darwent MJ,Paterson E,McDonald AJ,Tomos AD

    更新日期:2003-01-01 00:00:00

  • Interactive effects of water stress and xylem-limited bacterial infection on the water relations of a host vine.

    abstract::Xylella fastidiosa, a xylem-limited bacterial pathogen that causes bacterial leaf scorch in its hosts, has a diverse and extensive host range among plant species worldwide. Previous work has shown that water stress enhances leaf scorch symptom severity and progression along the stem in Parthenocissus quinquefolia infe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg046

    authors: McElrone AJ,Sherald JL,Forseth IN

    更新日期:2003-01-01 00:00:00

  • The role of phytochelatins in constitutive and adaptive heavy metal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes.

    abstract::Using the gamma-glutamylcysteine synthetase inhibitor, L-buthionine-[S,R]-sulphoximine (BSO), the role for phytochelatins (PCs) was evaluated in Cu, Cd, Zn, As, Ni, and Co tolerance in non-metallicolous and metallicolous, hypertolerant populations of Silene vulgaris (Moench) Garcke, Thlaspi caerulescens J.&C. Presl., ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf107

    authors: Schat H,Llugany M,Vooijs R,Hartley-Whitaker J,Bleeker PM

    更新日期:2002-12-01 00:00:00

  • KNAP2, a class I KN1-like gene is a negative marker of bud growth potential in apple trees (Malus domestica [L.] Borkh.).

    abstract::The determinism of bud bursting pattern along the 1-year-old shoot was studied at the molecular and morphological levels in the apple tree variety 'Lodi' which shows an acrotonic tendency. At the molecular level, the expression of KNAP2, which belongs to the class I KN1-like gene family, was studied. Measurements were...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf063

    authors: Brunel N,Leduc N,Poupard P,Simoneau P,Mauget JC,Viémont JD

    更新日期:2002-11-01 00:00:00

  • Chilling root temperature causes rapid ultrastructural changes in cortical cells of cucumber (Cucumis sativus L.) root tips.

    abstract::Examination of root tips from cucumber (Cucumis sativus L.) seedlings grown at 8 degrees C for varying periods ranging from 15 min to 96 h, showed marked changes in the ultrastructure of cortical cells within only 15 min of exposure. Greater parts of the cortex were affected with longer periods of exposure, but the se...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf071

    authors: Lee SH,Singh AP,Chung GC,Kim YS,Kong IB

    更新日期:2002-11-01 00:00:00

  • Genetic identification and genomic organization of factors affecting fruit texture.

    abstract::Fleshy fruits are an essential part of the human diet providing vital vitamins, minerals and other health-promoting compounds. The texture of the ripe fruit has a significant effect on quality and influences consumer acceptance, shelf-life, resistance, and transportability. The development of rational approaches to im...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erf087

    authors: Seymour GB,Manning K,Eriksson EM,Popovich AH,King GJ

    更新日期:2002-10-01 00:00:00

  • Independent induction of two blue light-dependent monovalent anion transport systems in the plasma membrane of Monoraphidium braunii.

    abstract::In the plasma membrane of the green alga Monoraphidium braunii there are at least two monovalent anion transport systems. One of them is specific for bicarbonate. This transport system is activated by blue light and its induction is triggered by a decrease in the external CO2 concentration. The second transport system...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf056

    authors: Mora C,Witt FG,Aparicio PJ,Quiñones MA

    更新日期:2002-09-01 00:00:00

  • The novel rice (Oryza sativa L.) gene OsSbf1 encodes a putative member of the Na+/bile acid symporter family.

    abstract::PCR-based differential screening was used to identify ethylene-induced genes in deep-water rice (Oryza sativa L.). One of the isolated cDNAs represented a novel protein, OsSBF1, with high homology to mammalian Na+/bile acid transporters and to sodium-dependent transporters from bacteria. One highly homologous protein ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf053

    authors: Rzewuski G,Sauter M

    更新日期:2002-09-01 00:00:00

  • Hyperosmotic stress-induced actin filament reorganization in leaf cells of Chlorophyton comosum.

    abstract::Actin filament (AF) organization was studied during the plasmolytic cycle in leaf cells of Chlorophyton comosum Thunb. In most cells the hyperosmotic treatment induced convex or concave plasmolysis and intense reorganization of the AF cytoskeleton. Thin cortical AFs disappeared and numerous cortical, subcortical and e...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf018

    authors: Komis G,Apostolakos P,Galatis B

    更新日期:2002-08-01 00:00:00

  • Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method.

    abstract::Rutin is an antioxidant with many interesting pharmacological effects. It can also be found in buckwheat (Fagopyrum esculentum Moench). UV radiation stimulates the activity of enzymes of the phenylpropanoid pathway and there is some evidence that it influences the rutin content in plants. The aim of the present resear...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf032

    authors: Kreft S,Strukelj B,Gaberscik A,Kreft I

    更新日期:2002-08-01 00:00:00

  • Ecophysiological analysis of genotypic variation in peach fruit growth.

    abstract::Cultivated varieties generally differ greatly from wild genotypes of the same closely related species. However, the processes responsible for these differences have not been elucidated. To analyse variations in fruit mass, fruit growth was characterized in a peach cultivar, a wild related species non-cultivated, and f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf001

    authors: Quilot B,Génard M,Kervella J,Lescourret F

    更新日期:2002-07-01 00:00:00

  • alpha4-Fucosyltransferase is regulated during flower development: increases in activity are targeted to pollen maturation and pollen tube elongation.

    abstract::alpha4-Fucosylation represents a final step of protein N- glycosylation. alpha4-fucosylated N-glycans are thought to be involved in cell-to-cell communication and recognition in primates and plants. Nevertheless, in the plant life cycle, the function of alpha4-fucosylation remains largely unknown. To gain an insight i...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.373.1429

    authors: Joly C,Léonard R,Maftah A,Riou-Khamlichi C

    更新日期:2002-06-01 00:00:00

  • Cloning and characterization of a phospholipase C from the C(4) plant Digitaria sanguinalis.

    abstract::As a PLC activity was implicated in the light transduction pathway that controls C(4) photosynthesis in Digitaria sanguinalis, a full length PLC cDNA (DsPLC2) was cloned. The proteins encoded by the two possible open reading frames were produced in Escherichia coli; they both harbour a PLC activity but with different ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Coursol S,Pierre JN,Vidal J,Grisvard J

    更新日期:2002-06-01 00:00:00

  • A comparison of the effects of IAA and 4-Cl-IAA on growth, proton secretion and membrane potential in maize coleoptile segments.

    abstract::The physiological activity of exogenous 4-Cl-IAA, as compared to IAA, was examined in maize coleoptile segments. It was found that in this model system 4-Cl-IAA is much more active in the stimulation of elongation than IAA. Simultaneous measurements of growth and external pH indicated that administration of either IAA...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.371.1089

    authors: Karcz W,Burdach Z

    更新日期:2002-05-01 00:00:00

  • Two early light-inducible protein (ELIP) cDNAs from the resurrection plant Tortula ruralis are differentially expressed in response to desiccation, rehydration, salinity, and high light.

    abstract::Tortula ruralis (Syntrichia ruralis) is a useful model system for the study of gene control in response to severe water deficit-stress. EST gene discovery efforts utilizing desiccated gametophytes have identified two cDNAs designated Elipa and Elipb with significant similarity to early light-inducible proteins (ELIPs)...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.371.1197

    authors: Zeng Q,Chen X,Wood AJ

    更新日期:2002-05-01 00:00:00

  • Pyramiding Mn-superoxide dismutase transgenes to improve persistence and biomass production in alfalfa.

    abstract::Expression of individual superoxide dismutase (SOD) transgenes improves environmental stress tolerance and biomass production in alfalfa (Medicago sativa L.). The objective of this study was to test the hypothesis that synergy exists between transgenic SOD stress-tolerance mechanisms, specifically that the simultaneou...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Samis K,Bowley S,McKersie B

    更新日期:2002-05-01 00:00:00

  • Variation in the k(cat) of Rubisco in C(3) and C(4) plants and some implications for photosynthetic performance at high and low temperature.

    abstract::The capacity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) to consume RuBP is a major limitation on the rate of net CO(2) assimilation (A) in C(3) and C(4) plants. The pattern of Rubisco limitation differs between the two photosynthetic types, as shown by comparisons of temperature and CO(2) responses o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.369.609

    authors: Sage RF

    更新日期:2002-04-01 00:00:00

  • Engineering for drought avoidance: expression of maize NADP-malic enzyme in tobacco results in altered stomatal function.

    abstract::Water is a principal limitation to agricultural production during drought and in arid regions of the world. Mechanisms that plants use to cope with drought can be grouped into two different strategies: drought tolerance and drought avoidance. Previous efforts toward engineering plants for improved performance during d...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.369.699

    authors: Laporte MM,Shen B,Tarczynski MC

    更新日期:2002-04-01 00:00:00

  • Modulation of nitrate reductase: some new insights, an unusual case and a potentially important side reaction.

    abstract::The mechanism of the post-translational modulation of nitrate reductase activity (NR, EC 1.6.6.1) is briefly summarized, and it is shown that by this mechanism nitric oxide production through NR is also rapidly modulated. New and partly unexpected details on the modulation mechanism have been obtained by using immunol...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jexbot/53.370.875

    authors: Kaiser WM,Weiner H,Kandlbinder A,Tsai CB,Rockel P,Sonoda M,Planchet E

    更新日期:2002-04-01 00:00:00

  • The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops.

    abstract::This short review outlines the central role of glutamine synthetase (GS) in plant nitrogen metabolism and discusses some possibilities for crop improvement. GS functions as the major assimilatory enzyme for ammonia produced from N fixation, and nitrate or ammonia nutrition. It also reassimilates ammonia released as a ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jexbot/53.370.979

    authors: Miflin BJ,Habash DZ

    更新日期:2002-04-01 00:00:00

  • Short-term metabolic responses of soybean root nodules to nitrate.

    abstract::Soybean (Glycine max L. Merr.) plants exposed to 10 mM KNO(3) for a 4 d period were used to test the correlation between nitrogenase activity, gene expression and sucrose metabolism. Nitrate caused the down-regulation of sucrose synthase (SS) transcripts within 1 d, although a decline in nodule SS activity and an incr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.368.423

    authors: Gordon AJ,Skøt L,James CL,Minchin FR

    更新日期:2002-03-01 00:00:00

  • Organization of cell walls in Sandersonia aurantiaca floral tissue.

    abstract::Visual symptoms of the onset of senescence in Sandersonia aurantiaca flowers begin with fading of flower colour and wilting of the tissue. When fully senescent, the flowers form a papery shell that remains attached to the plant. The cell walls of these flowers have been examined to determine whether there are wall mod...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.368.513

    authors: O'Donoghue EM,Somerfield SD,Heyes JA

    更新日期:2002-03-01 00:00:00

  • Long-distance signalling from roots to shoots assessed: the flooding story.

    abstract::Several kinds of signal may be generated when roots are exposed to an environmental stress. Some, but not all, are conveyed to shoots in the transpiration stream. Principles are summarized that may help establish experimentally the presence or intensity of root signals transported by transpiration. In many dryland spe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jexbot/53.367.175

    authors: Jackson MB

    更新日期:2002-02-01 00:00:00

  • Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: identification, characterization and immunolocalization of a putative taproot storage glycoprotein.

    abstract::In taproot of oilseed rape (Brassica napus L.), a 23 kDa polypeptide has been recently identified as a putative vegetative storage protein (VSP) because of its accumulation during flowering and its specific mobilization to sustain grain filling when N uptake is strongly reduced. The objectives were to characterize thi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.367.265

    authors: Rossato L,Le Dantec C,Laine P,Ourry A

    更新日期:2002-02-01 00:00:00

  • In situ staining of activities of enzymes involved in carbohydrate metabolism in plant tissues.

    abstract::A powerful technique is described to localize the activities of a range of enzymes in a wide variety of plant tissues. The method is based on the coupling of the enzymatic reaction to the reduction of NAD and subsequent reduction and precipitation of nitroblue tetrazolium. Enzymes that did not reduce NAD could be visu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.367.361

    authors: Sergeeva LI,Vreugdenhil D

    更新日期:2002-02-01 00:00:00

  • Use of mutants to study long-distance signalling in response to compacted soil.

    abstract::When plants encounter compacted soil, stomatal closure occurs and shoot growth slows. These responses occur in the absence of detectable changes in foliar water status. The use of genotypes with a reduced capacity to synthesize either ABA or ethylene has provided convincing evidence that ABA is responsible for providi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:

    authors: Roberts JA,Hussain A,Taylor IB,Black CR

    更新日期:2002-01-01 00:00:00

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